BioDefense
Food Security Testing
Water Security Testing
Mutation Discovery
  - LightScanner
  - LightScanner 32
  - LightScanner Master Mixes
  - LCGreen Plus Melting Dye
  - LightScanner Assays
  - Custom Probes & Primers
  - Reagents, Buffers & Capillary Tubes
Genotyping
FilmArray
Mutation Discovery Using High Resolution Melting

Mutations in PCR products are detected by the presence of heteroduplexes optimally detected by our LCGreen® Plus dye. Variations are identified by changes in the shape of the melting profile compared to a reference sample. Hi-Res Melting® (HRM) on the LightScanner® is also being used for mutation discovery in tumor samples, yielding information on mutations present in low allele fractions (as low as 5%) and allelic imbalances between tumor and matched normal samples.

Use the links below to learn how your research can get there faster with our complete line of products.
Instrument

LightScanner Instrument
LightScanner® System

  • Hi-Res Melting Instrument
  • 96- or 384-well plate system
  • Results in less than 5 minutes
  • Software automatically analyzes each sample and defines the standards and variants

LightScanner 32 System
LightScanner 32 System

  • Fastest real-time PCR combined with the most accurate high resolution melting system
  • 32-sample carousel
  • Analysis time less than 30 minutes
Chemistry

LightScanner Master Mix
LightScanner Master Mix

  • A pre-optimized solution
    specifically designed to deliver superior PCR performance for Hi-Res Melting applications on the LightScanner Instrument.

LightScanner Master Mix
Genotyping Master Mix

  • A pre-optimized Master Mix specially designed for genotyping experiments not requiring an enzyme with exonuclease activity.

High Sensitivity Master Mix
High Sensitivity Master Mix

  • The LightScanner® High Sensitivity Master Mix is a specialized master mix containing LCGreen Plus® dye and internal temperature calibrators.

Warfarin Genotyping Reagents
Warfarin Genotyping Reagents

  • Our Warfarin Genotyping Reagents (ASR) provide a fast and effective solution for genotyping.

LCGreen + Melting Dye
LCGreen® Plus+ Melting Dye

  • LCGreen dye is specifically designed for Hi-Res Melting curve analysis to detect DNA sequence variants (mutations, polymorphisms, etc).

LCGreen + Melting Dye
Reagents, Buffers and Capillary Tubes
Services

LightScanner Assay Protocols
LightScanner Assays

  • Free assay design protocols that have been optimized for Hi-Res Melting.

IT BioChem - Custom Probes, Primers and Melting Dyes
Custom Probes & Primers

  • Order custom designed probes, primers and melting dyes for DNA amplificaiton, mutation and detection.
  • ASR grade oligos.
  • Manufactured under cGMP.
 
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