BioSurveillance Systems
Food & Water Security Testing Systems
Hi-Res Melting for Mutation Discovery
  - LightScanner
  - LightScanner 32
  - LightScanner Master Mixes
  - LCGreen Plus Melting Dye
  - LightScanner Assays
  - Reagents, Buffers & Capillary Tubes
Clinical Diagnostic System
Mutation Discovery Using High Resolution Melting

Mutations in PCR products are detected by the presence of heteroduplexes optimally detected by our LCGreen® Plus dye. Variations are identified by changes in the shape of the melting profile compared to a reference sample. Hi-Res Melting® (HRM) on the LightScanner® is also being used for mutation discovery in tumor samples, yielding information on mutations present in low allele fractions (as low as 5%) and allelic imbalances between tumor and matched normal samples.

Use the links below to learn how your research can get there faster with our complete line of products.
Instrument

LightScanner Instrument
LightScanner® System

  • Hi-Res Melting Instrument
  • 96- or 384-well plate system
  • Results in less than 5 minutes
  • Software automatically analyzes each sample and defines the standards and variants

LightScanner 32 System
LightScanner 32 System

  • Fastest real-time PCR combined with the most accurate high resolution melting system
  • 32-sample carousel
  • Analysis time less than 30 minutes
Chemistry

LightScanner Master Mix
LightScanner Master Mix

  • A pre-optimized solution
    specifically designed to deliver superior PCR performance for Hi-Res Melting applications on the LightScanner Instrument.

LCGreen + Melting Dye
LCGreen® Plus+ Melting Dye

  • LCGreen dye is specifically designed for Hi-Res Melting curve analysis to detect DNA sequence variants (mutations, polymorphisms, etc).

LCGreen + Melting Dye
Reagents, Buffers and Capillary Tubes
Services

LightScanner Assay Protocols
LightScanner Assays

  • Free assay design protocols that have been optimized for Hi-Res Melting.
As of May 1, 2012 we have discontinued our Genotyping Master Mix Kit, High Sensitivity Master Mix Kit and Warfarin Genotyping Reagents. We continue to support these items online as well as through our superior customer service team. If you have any questions or concerns, please contact our support team.
 
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